StemSure® Gelatin Solution is a coating agent that can be used for cell culture dishes and multi-well plates.
It is composed of gelatin derived from porcine skin, and is prepared with D-PBS (-).
Multi-well plates were coated with StemSure® Gelatin Solution, and feeder sells were seeded onto the coated wells. Human iPS cell line 201B7 was cultured on the feeder cells, and the morphology of the iPS cells and positivity for ALP staining were examined.
D-MEM/Ham's F-12 + 20% StemSure® Serum Replacement + 2 mmol/L L-Glutamine + 1 x MEM Non-essential Amino Acids + 0.1 mmol/L StemSure® 2-Mercaptoethanol +1 x Penicillin-Streptomycin + 5 ng/mL bFGF
Cloning efficiency for murine ES cell line D3 was compared for cells cultured on multi-well plates coated with StemSure® Gelatin Solution and those cultured on plates coated with an equivalent material from company A.
Murine ES cell line D3 was cultured on a multi-well plate coated with StemSure® Gelatin Solution. After 16 passages, cells stained positive for markers of undifferentiated cells (Oct3/4, Sox2, Nanog).
Murine ES cell line D3 was cultured for multiple passages on multi-well plates coated with StemSure®Gelatin Solution. Cells were then injected subcutaneously in immunocompromised mice. Teratomas formed subcutaneously, and various tissue types including nerve tissue (derived from ectoderm), cartilage tissue (derived from mesoderm), and luminal structures with ciliated epithelium (derived from endoderm) were found in the teratomas.